| 质粒名称: | pLenti CMV GFP Neo (657-2) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17447 |
| 存储实验室: | Eric Campeau, Paul Kaufman |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8068 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP,enhanced GFP |
| 物种来源: | -- |
| 插入大小: | 719 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | Sal I (未破坏) |
| 5' 测序引物 | CMVforw |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti CMV GFP Neo (657-2) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17447 ; http://n2t.net/addgene:17447 ; RRID:Addgene_17447) |
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pLenti CMV GFP Neo (657-2) 8798 bp ds-DNA circular SYN 09-NOV-2020
DEFINITION 3rd gen lentiviral eGFP expression vector, CMV promoter, Neo.
ACCESSION .
VERSION .
KEYWORDS pLenti CMV GFP Neo (657-2)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8798)
AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
Campisi J, Yaswen P, Cooper PK, Kaufman PD.
TITLE A versatile viral system for expression and depletion of proteins in
mammalian cells.
JOURNAL PLoS One. 2009 Aug 6;4(8):e6529.
PUBMED 19657394
REFERENCE 2 (bases 1 to 8798)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 9, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8798
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 58..162
/gene="bla"
/label=AmpR promoter
CDS 163..1023
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(381..400)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1194..1782
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1683..1702
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1936..1953
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2070..2091
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2106..2136
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2144..2160
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2149..2171
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2168..2184
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2168..2184
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2203..2223
/label=T3
/note="T3 promoter, forward primer"
promoter 2205..2223
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 2251..2477
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 2478..2658
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2705..2830
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3323..3556
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 4052..4168
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 4191..4494
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4495..4698
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4649..4669
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 4695..4719
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 4788..4797
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4794..5513
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4839..4860)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5100..5119)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5447..5468
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5529..6117
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5582..5602)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6000..6011)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 6160..6659
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(6195..6218)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 6592..6611
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 6673..7476
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6736..6755)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7346..7365
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
LTR 7567..7800
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7872..7993
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7909..7928)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7963..7982
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 8033..8168
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8095..8114
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(8188..8207)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8189..8207)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8217..8234)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8217..8233)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8226..8248)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 8375..32
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8462..8481)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8672..8693
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
61 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
121 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
181 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
241 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
301 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
361 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
421 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
481 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
541 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
601 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
661 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
721 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
781 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
841 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
901 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
961 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
1021 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
1081 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
1141 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
1201 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
1261 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
1321 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
1381 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
1441 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
1501 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
1561 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
1621 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
1681 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
1741 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
1801 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
1861 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
1921 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
1981 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
2041 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
2101 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
2161 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
2221 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
2281 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
2341 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
2401 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
2461 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
2521 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
2581 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
2641 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
2701 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
2761 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
2821 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
2881 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
2941 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
3001 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
3061 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
3121 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
3181 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
3241 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
3301 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
3361 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
3421 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
3481 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
3541 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
3601 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
3661 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
3721 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
3781 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
3841 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
3901 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
3961 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
4021 tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt
4081 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
4141 aaaaacaaat tacaaaattc aaaattttat cgataagctt gggagttccg cgttacataa
4201 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
4261 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
4321 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
4381 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
4441 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
4501 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
4561 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
4621 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
4681 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
4741 tgttttgacc tccatagaag acaccgactc tagaggatcc accggtcgcc accatggtga
4801 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
4861 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
4921 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
4981 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
5041 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
5101 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
5161 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
5221 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
5281 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
5341 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
5401 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
5461 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
5521 gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
5581 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
5641 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
5701 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
5761 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
5821 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
5881 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
5941 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
6001 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
6061 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
6121 gaattctgca gatatccgga ctagtgatct aattctaccg ggtaggggag gcgcttttcc
6181 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
6241 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
6301 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
6361 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
6421 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
6481 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
6541 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
6601 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
6661 cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
6721 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
6781 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
6841 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
6901 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
6961 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
7021 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
7081 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
7141 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
7201 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
7261 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
7321 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
7381 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
7441 atcgccttct atcgccttct tgacgagttc ttctgagggg atccgtcgag ggcccggtac
7501 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
7561 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg
7621 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
7681 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
7741 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
7801 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
7861 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
7921 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
7981 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
8041 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
8101 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
8161 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
8221 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
8281 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
8341 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
8401 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
8461 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
8521 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
8581 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
8641 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
8701 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
8761 tggttaaaaa atgagctgat ttaacaaaaa tttaacgc
//
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